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Role of MAPK and PI3K in Nerve Growth(2007/9/17 21:36:22)
ABSTRACT: ObjectiveTo investigate the role of mitogenactivated protein kinase(MAPK) and phosphoinositide 3kinase(PI3K)/Akt pathways in differentiation of PC12 cells induced by nerve growth factor(NGF) from venom. Methods The phosphorylation of p44/p42MAPK and Akt were detected by Western blot. The role of MAPK and PI3K in the cellular action of PVC12 cells induced by NGF were assessed by use MAPK and PI3K specific inhibitor PD98059 and LY294002 respectively. Results NGF from cobra venom can stimulate p44/p42MAPK and Akt phosphorylation and induce the differentiation of PC12 cells from 10 ng/mL to 1000 ng/mL. The differentiation of PC12 cells induced by NGF was inhibited when MAPK or PI3K pathway was abolished by the specific inhibitor PD98059 and LY294002. Conclusion The differentiation of PC12 cells by NGF from cobra venom relates to p44/p42MAPK and PI3K/Akt pathway.
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